Abstract:

With immature embryos of Citrus grandis‘Guanximiyou’ as material, the effect of the three factors, enzyme treating time, pycnosis treating time in ice water and dyeing, were studied in the process of single chromosome microdissection technique. The resluts indicated that the ideal effects had been proved with enzymatic hydrolysis of mixing 2% cellulose enzyme and 0.5% pectic enzyme for 40～60 min, then pycnosis treatment in ice water for 20～30 min after post-hypotonic treatment, and carbol fuchsin solution dyeing. Using this technique, the chromosome images were clear with satisfying background, each chromosome separation and extension adequately, it is suitable for next research for single small chromosome microdissection.