Abstract: Peach (Prunus persica L.cv.Okubao) fruits around 56 days after anthesis were adopted in this experiment. The total protein was extracted and isolated by immobilized pH gradient（IPG）based on 2-DE routine. 1273±101 and 1292±115 protein spots were detected respectively from 2-DE patterns of mesocarp and putamen. Many spots of aggregate matched protein spots were present in both patterns of mesocarp and putamen, and 30 spots showed 2-fold（t-test, p<0.05）up-regulated on quantitative appearing an increase in spot intensity and maximum abundance in putamen than mesocarp , in addition 35 protein spots expressed 2-fold（t-test, p<0.05）down-regulated. 10 spots were just observed in mesocarp’s pattern, whereas 18 spots was only found in putamen.