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Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (8): 226-229.

Special Issue: 生物技术； 农业生态

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Application of enzymatic kinetics method in resistance monitoring of striped flea beetle, phyllotreta striolata (Coleoptera: Chrysomelidae)

Zheng Lizhen1,2, Fu Jianwei1,2, Li Jianyu1, Lin Zeyan2, Zhan Zhixiong1   

  • Received:2009-10-13 Revised:2009-11-18 Online:2010-04-20 Published:2010-04-20

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Abstract:

Abstract: Frequency distributions of general esterase and acetylcholinesterase in striped flea beetle，phyllotreta striolata，from 4 regions were detected with enzymatic kinetics method in a microplate reader, and were compared with the insecticide susceptibility tested by leaf dipping methods. The results indicated that the former method was a rapid detection of insecticide susceptibility of striped flea beetle by analyzing individual number on the general esterase with an activity of more than 3.2⊿D(450nm)?min-1?insect-1, while acetylcholinesterase less than 0.016⊿D(405nm)?min-1?insect-1.