Abstract:

Abstract: Our Black - grained Wheat contains black endosperm and rich water-soluble pigment, which has been studied both at home and abroad.There is almost no study on free radical scavenging effect of these pigments in Black - grained Wheat by DPPH. Using Chinese Spring and Vc as the control, We evaluate free radical scavenging and anti-aging effect of pigment crude extract in Black- -grained Wheat by DPPH. The result shows that pigment in Black - grained Wheat has dose-dependent effect on free radical scavenging, The ability of free radical scavenging and anti-aging of the pigment crude extract from Black - grained Wheat was greater than those of Chinese Spring, but lower than those of Vc. EC50 ( 50 % the initial DPPH ? concentration) of Chinese Spring、Black- -grained Wheat3-2、3-3、3-4、3-11、3-13、3-33、3-36、3-37、3-39、10-38 and Vc was 689.05、39.89、71.51、58.57、48.13、33.99、105.27、62.15、30.97、35.15、35.09、0.196 mg/ml DPPH ?respectively , and the antiradical efficiency (AE) was 0.15×10-2、2.51×10-2、1.4×10-2、1.7×10-2、2.08×10-2、2.94×10-2、0.95×10-2、1.61×10-2、3.23×10-2、2.84×10-2、2.85×10-2、510×10-2.All of this lay a foundation for further study, exploit and utilize pigment in Black - grained Wheat.