Abstract:

【Objective】To investigate the effects of insulin-like growth factor 1 (IGF1)on the proliferation of sika deer antler mesenchymal layer cells from different passages in vitro. 【Method】The mesenchymal layer cells were isolated from sika deer antlers of growing 90 day and cultured. The second, fifth and eighth population cells were incubated with 0, 1, 3, and 10 nM IGF1. Thymidine incorporation assay was applied after 24 hours of culture respectively to determine the radioactivity content of protein synthesis. 【Results】The average and maximal radioactivity content of protein synthesis value of the proliferation of cells from growing 90 day’s antler in the experimental group were the highest in the second population cells (32146 and 35973 DPM/mg respectively), decreased in the fifth population cells materially (2781 and 3206 DPM/mg), and decreased continuously in the eighth population cells (489 and 736 DPM/mg) and there was no significant difference compared with the control group. 【Conclusion】The sensitivity of sika deer antler mesenchymal layer cells to IGF1 varies from various passages. There was no difference in sensitivity to IGF1 in the eighth population cells at 90 days growing period of antler in vitro.