Abstract:

High quality DNA was obtained from Chinese Kale (Brassica alboglabra Bailey） leaf by the improved method of CTAB．Based on the common RAPD reaction program and the adjusting experiments，the optimal PCR system（25μl total volumes）contains：5mmol/LMgCl2 2.0μL, 10×PCR Buffer 2.5μL, 2.5mmol/L dNTPs2.0μL, 5U/μLTaq DNA polymerase 0.25μL, 5μmol/ L primer1.2μL、genomic DNA 25ng and ddH2O 12.25μl. The optimal amplification program as follows：94℃ for 5 min；40 cycles at 94℃ for 1 min，36℃ for 1 min and 72℃ for 2 min；72℃ for 10 min at last.