Abstract:

At present, reported at detection of HMW-GS 1Dx5 molecular markers, the marker,composed of three primers Dx-F, Dx5-F and Dx-R, is a specific co-dominant PCR markers for 1Dx5 subunit,and has no non-specific amplicon.It was strongly recommended to be used in wheat molecular assiatant selection (MAS) breeding for good bread-marking quality. The experimental use of this pair of primers by PCR technology for 221copies of a parental wheat gene 1Dx5 subunit. The results showed that the detection of 35 copies of Materials Materials 1Dx5subunit carried, representing the total number of measured Materials16.3%, much lower than the frequency of foreign genes (about 85%).