Abstract:

IEF- SDS- PAGE two- dimensional gel electrophoresis (2- DE) technology was used to separated proteins from ungerminated seed and embryo buds of germinated seed at specific stages of 1 d、2d、3d、4d in rice(Oryza sativa L.)Jindao 1. The results showed that on the 2-DE gels stained by coomassie brilliant blue，PDQuest image software detected about 215、201、187、162、132 protein spots， of which 22 spots show more than 2.5-fold changes in abundance among ungerminated seed and embryo buds of germinated seed at specific stages of 1 d、2d、3d、4d in rice(Oryza sativa L.)Jindao 1. These 12 of 22 protein spots treated by tryptic in-gel digestion were characterized by matrix-assisted laser -desorption／ionization time of flight mass spectrometry (MALD I-TOF -MS) and bioinformatics analysis,peptidemass fingerprintings of all were obtained，of which 6 differential proteins were elementarily identified as thioredoxin peroxidase，heat shock protein, ATP synthase, LEA protein 12, nucleoside diphosphate kinaseⅠ(NDK Ⅰ)(NDP kinase Ⅰ)(NDPK Ⅰ)and glyoxalase I respectively. The potential functions of these proteins during seed germination were discussed．