Abstract:

Bacillus thuringiensis strain HYW-8 was isolated from the soil in the city of Yichun by antibiotic method.It produced small diamond-shaped crystals. It was identified that contained cry1A,cry1E gene and an unknown cry2 gene by PCR-RFLP.Bioassay showed that the strain HYW-8 had high insecticidal activity to Hyphantria cunea.The LC50 was 0.74ug/ml,which was lower than the standard strain of Bt subsp kurstaki strain HD-1.We suggested that the potential of the strain HYW-8 for used in microbial control of the larvae of Hyphantria cunea.