Abstract:

The aim is to observe and analyze the infection process of E.coli by bacteriophag Bp7 systematically by TEM and its lysis spectrum. The results show that, Bp7 can lyse 14 in 40 strains of E.coli and its lysis rate is 35%. Bp7 has a hexagonal head with diameter of 93nm and connected with a flexible tail which mean length is 106nm. The tail has tail sheath. Bp7 adsorbed on the rod-shaped E.coli via its tail wire. In the first 30min, the infection is in the hidden phase, and maturity phase appears at 45mpi (minute post infection), release period is at 60mpi, and a lytic cycle is about 60min. This study provides a theoretical basis for bacteriophag as an alternative to antibiotics in the treatment of bacterial diseases.