Abstract:

Abstract：The current study taking Fritillaria ussuriensis Maxim. as experiment material, and the research objective were measuring amylas isozyme and activity in cultures of Fritillaria ussuriensis Maxim. during different stage of tissue culture.The results showed that AMY isozyme produced differential enzyme band of Fritillaria ussuriensis α-amylase at Rf=0.167 and light blue R-amylase was expressed during the four phases at Rf=0.250 in cultures during different stage of tissue culture. While enzyme band of β-amylase was only expressed during bulblet expanding. Total amylase activity showed decrease - increase - decrease trend in cultures. The change trend of α-amylase and total amylase was similar, activity of α-amylase was always highter than β-amylase during different stage.