Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (4): 30-32.

Special Issue: 生物技术 马铃薯

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Genomic DNA Extraction in Cassava by Modified CTAB Method

  

  • Received:2009-11-25 Revised:2009-12-02 Online:2010-02-20 Published:2010-02-20

Abstract:

Abstract: In this paper,genomic DNA extraction in cassava by modified CTAB method,the extracted products had the clear band and without tailing bands which could be observed through agar gel electrophoresis. The results show that the concentrations were between 710.0 ng/μl~967.5 ng/μl,and the values of OD260/OD280 were between 1.73~1.92. The modified CTAB method had characteristics of simple、rapid and effective,the genomic DNA extracted had high quality and purity,it could be used for SSR、 ISSR and other molecular markers.