Abstract:

Tapping panel dryness (TPD) is one of the most serious threats to natural rubber production. It exists in almost every rubber plantation country and causes serious damage to natural rubber production. It is well known that elucidation of molecular mechanism of TPD is prerequisite for taking effective measures to prevent TPD. To elucidate the molecular mechanism of TPD, the expression profiles of lutoids proteins from TPD and healthy rubber trees was analyzed by 2-DE method and PDQuest7.40 software. After digesting peptides differentially expressed with enzymes, the peptide mass fingerprints were obtained with MALDI-TOF-MS, and then identified by searching SWISS-PROT and NCBI nr database. In this research, a total of 13 peptides differentially expressed were identified. Compared with the expression patterns from healthy tree, 11 and 2 proteins were upregulated and downregulated in TPD tree, respectively. These proteins might play important roles in TPD process.