Abstract:

Abstract: Under low-temperature stress, the changes of protein content and component in leaves of annual Toona Sinensis provenances seedlings, Xixia of Henan, Nanjing of Jiangsu, Xiapu of Fujian, were studied. The results indicated that the low-temperature stress could change the content and component in leaves of Toona Sinensis provenances seedlings. Different provenances had different changes under different temperatures. So did the same provenance under different stress period. SDS-PAGE electrophoresis indicated that low-temperature stress could induce specific proteins’ expression. The protein contents of Xixia of Henan and Nanjing of Jiangsu provenances, whose cold resistance were stronger, tended to change as ‘high-low-high’ pattern during one to four days under -5℃. Proteins were induced highly in the first day, and the band became deep. During the second and the third days, it’s the opposite. While in the forth day, it began to express more again. And there were two specific proteins expressed, the molecular weights were 27.6 KD and 22.5 KD for the two provenances respectively. The protein content of Xiapu of Fujian, whose cold resistance were weaker, tended to increase gradually, and had no new proteins appeared. By electrophoresis of SDS-PAGE, the changing of the color of the protein bands changed almost the same with the protein contents.