Abstract:

In order to discover the characteristics of blood coagulation for Chinese soft-shelled turtle, some general blood clotting tests have been done, for example, the recalcified whole blood clotting time（CT）test, the plasma recalcification time (RT) test, the activated plasma clotting time (ACT) test, the activated partial thromboplastin time (APTT) test, the prothrombin time (PT) test and the thrombin time (TT) test, the samples of whole blood or plasma were treated with citrate and differently diluted 1-, 2-, 3-fold with ion-free water. The results prove that: this paper firstly reported the ACT, the APTT and the PT for Chinese soft-shelled turtle were 332.65 s, 147.75 s, 50.61 s, which are different from mammals, however, the CT, the CT and the TT are no difference between Chinese soft-shelled turtle and mammals; 2) the cell-free plasma of Chinese soft-shelled turtle uncoagulates within 10 min. , but the diluted cell-free plasma of Chinese soft-shelled turtle coagulate; 3) the ACT and the APTT are extremely significant difference (P＜0.01) between the diluted plasma and the control group for Chinese soft-shelled turtle, however, the PT is nonsignificant difference (P＞0.05) between the diluted plasma(1:1, 1:2) and that of the control group. Conclusions: the diluted cell-free plasma of Chinese soft-shelled turtle has coagulated, primarily by influencing the intrinsic pathway but has nothing to do with the extrinsic pathway.