Abstract:

Mycobacterium tuberculosis CFP10 gene sequence was designed and synthesized primer 1 pairs to build a primer containing the sequence of the recombinant plasmid was amplified as a positive standard, the establishment of a nucleic acid of CFP10 Detection SYBR Green Ｉreal-time PCR method. Test results showed a good linear relationship between the method, standard curve correlation coefficient reached 0.997, the initial template for the detection threshold of 1 × 101copies/μL, than the conventional PCR method of the high 100 times. Show that the established real-time PCR detection method is sensitive, specific, reproducible and can be used to detect the pathogen Mycobacterium tuberculosis CFP10 and quantitative analysis.