Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (11): 227-230.

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Isolated culture and Establishment of Regeneration System Of Sophora japonica in Higher Efficiency

Zhang Xianyun   

  • Received:2009-12-31 Revised:2010-01-15 Online:2010-06-05 Published:2010-06-05

Abstract:

The paper studied the isolated culture and regeneration system of Sophora japonica using its shoots, and discussed the effects of different hormone combination on asepsis bud, callus, differentiation, reduplication and rooting. The results show MS+ BA1.0mg/L +NAA 0.1mg/L is optimum medium for induction of asepsis bud, callus; differentiation and reduplication; MB+BA1mg/L+NAA0.1+30g/L sucrose +8g/L agar is optimum medium for differentiation; 1/2MS+ NAA0.3mg/L is the optimum medium for rooting.