Abstract:

The orthogonal design was employed to optimize SRAP-PCR amplification .The results showed that the order of factors which affect on the result of PCR are Taq polymerase ,primers ,dNTPs , Mg2+ and DNA . A suitable SRAP-PCR system for Rose rugosa is that total 25μL reaction system containing 2.0 mmol/L Mg2+ , 0.20 mmol/L dNTPs , 1.5U Taq polymerase , 0.25 μmol/L primers and 1.0 ng/μl DNA. Use other primers and DNAs to test the system , the results showed that the system can be used in Rose rugosa.