Abstract:

The lengths of open reading frame (ORF)100 and ORF29-TrnCGCA, the intronic sequence of rps16 and the transcribed spacer of TrnTUGU-TrnLUAA in chloroplast of dual purpose genic male sterile line rice are studied using indica types and japonica types as controls. The results show that the lengths of ORF100 and ORF29-TrnCGCA in Zhu 1S, Lu 18S, Zhu 77S, H628S, 88S, Baitianlu S, G0543S, G03S, 1103S and W6154S are similar to those of typical indica, the others are similar to those of typical japonica. There are four special indica/japonica markers and the type of all dual purpose genic male sterile line rice based these special indica/japonica markers are same with the differentiation by ORF100 and ORF29-TrnCGCA. The materials such Zhun S, 810S, Zhongxin S, C185S and Peiai 64S those have japonica-type blood have more superiority than others based their quantity and quality of combination. Therefore, using dual purpose genic male sterile lines with japonica -type blood is an effective way to improve production of hybrid rice.