Abstract: Using G.barbadense G.thurberi and G. hirsutum crossing through distant hybridization of cotton, By dropping GA3(50ppm) and NAA(40ppm) on the pollinated flower,hybrid embryo culture,and in Vitro Colchicine treatment ,backcrossing to the hybrid , through continual selecting and breeding ,cultivate cotton new variety ShiYuan 321. It’s seed yield and lint yield and prefrose lint yield is the first of all the test varieties in the cotton regional test for the Yellow River Valley in 1993-1994. Its mean prefrost lint yield were 885kg.hm-2,19.7% higher than those of control “Zhongmiansuo”12 respectively ,it is the largest variety of prefrost lint yield than the control in the cotton regional test for the Yellow River Valley between 1982~2000.