Abstract:

Biological, physiological and biochemical characteristics were studied between procymidone-resistant mutants and their parent sensitive strains of Sclerotinia sclerotiorum. The results showed the carbon-nitrogen source and pH value were different between the mutants and the parent strains. The mutants and parent strains grew fastest respectively when carbon-nitrogen source and pH value were lactose, non-nitrogen, 6-7 and lactose, peptone and 5. Both grew similar in different temperatures. On the same osmotic stress (dextrose concentration), the mutants had more sensitive to osmotic stress than parent strains. The parent strains and mutants respectively were sensitive to low (1%) and high (> 4%) osmotic stress. Relative conductivity of the mutants was always higher than that of parent strains in same treat time. Relative conductivity of both rose with extension of treat time. Respectively treated with hunger and procymidone in different concentrations, the activity of PAL (phenlalanine ammona-lyase) of both increased, but the mutant had higher PAL activity than its present strain. It indicated that resistance of the mutants was related to its osmotic regulation, electrolyte leakage and correlate enzyme activity.