Abstract:

Abstract: This study was set up to find the effect on the induction effect of axillary buds of clone DH3229 of E.urophylla×E.grandis with the use of table concentrator and osmotic agent, concentration of colchicine solution and treatment time. The results show that every of them including the use of table concentrator and osmotic agent, concentration of colchicine solution and treatment time can significantly effect the induction rate of polyploid. Axillary buds can reach the highest induction rate under the condition of 0.2% colchicine solution and 24 hours’ treatment with the use of table concentrator and osmotic agent. There are no acquired polyploid plants if the treatment concentration was lower than 0.05%.The mortality of axillary buds after the treatment would steeply rise which could lead to the decrease of induction rate.