Abstract:

the variety of Canarium album Reausch was used as material, Optimization of SRAP-PCR system was studied with L16（45）orthogonal design of five factors (Taq polymerase, Mg2+, DNA template, dNTPs and primer) and the DNA of 11 varieties were amplified with the optimized SRAP-PCR system. The results showed that in 20μl amplification system contained 1.5U Taq DNA polymerase, 2.5mmol/L Mg2+, 60ng genomic templates DNA, 0.2mol/L dNTPs, and 0.15μmol/L Primer, the result of SRAP-PCR was best for Canarium album Reausch. Seven polymorphic bands were amplified with one primer pair Me5-Em2 through the optimized SRAP-PCR system in 11 varieties of Canarium album Reausch.