Abstract: Abstract: 【Objective】 antibacterial activity of mangrove soil actinomycetes of the separation and identification. 【Methods】 Gao on the 1st medium prepared with sea water used to dilute the separation of soil samples collected from isolated and purified by anti-bacterial screening tests, from Beihai, Guangxi Mangrove high-salt soil samples was isolated actinomycetes BH0954 The fermentation product of strain has a strong antimicrobial activity. The strain grew well on medium Gao on the 1st, and the optimum growth temperature 28 ℃, optimum growth pH of 7.0 to observe its morphological characteristics and physiological and biochemical characteristics; withdraw their total genomic DNA, using universal primers to their 16s rDNA PCR amplification, amplified products, DNA sequencing. 【Results】 based on their morphological characteristics, physiological and biochemical characteristics and 16S rDNA gene sequence-based phylogenetic analysis, BH0954 initially was identified as the genus Streptomyces Sendai kinds of effective publication of Streptomyces (Streptomyces sindenensis) variants. 【Conclusion】 mangrove soil actinomycetes BH0954 has antibacterial activity, this paper's findings for the further development and utilization of mangroves in Guangxi soil actinomycetes resource basis.