Abstract: With regard to the biodiversity of living things, FishBase is one of the largest databases in scope and extension .Up to now, FishBase has collected information on 31 000 fish species, covering their biology, ecology, taxonomy, population dynamics, genetics, physiological characteristics and economic values, bearing a high scientific and practical value. China became the ninth consortium member in 2007. The main function and structure of FishBase are summarized and analyzed in this paper. Different retrieval ways are also introduced, with an aim to assist research institutes and related enterprises with retrieval techniques and to provide technological support for the aquaculture industry. It is expected that the database would be better and more effectively used by research institutes and enterprises.