Abstract:

For optimizing ISSR-PCR reaction system of Procambarus clarkii, the single factor experiment included three factors, such as Taq DNA polymerse, dNTPs and Mg2+, to five different concentrations were set to study the effect of these variations in ISSR-PCR amplification in this research. In conclusion the reaction system was optimized as below：1 ?L 10xTaq Buffer, 0．6 mmol/L dNTPs，0.6 U Taq DNA polymerase, 1.8 mmol/L MgCl2, 20 ng DNA，0.8 ?mol／L primer and with total 10 ?L reaction solution．