Abstract: Herbicide widely application have solved the main problem of weed management in crop fields, while weed-resistance increasingly became a threat to herbicide application and crop production. Now there were many works on resistant-weeds distribution, biological characteristics, mechanisms, identification techniques and systematic management. Of these, identification of herbicide-resistance play an important role, and it was the fundament of system management In this present review we introduced some methods of herbicide-resistance identification, such as the whole plant assays in greenhouse, the biochemistry assays and the molecular techniques, while we analyzed the advantage and disadvantage of these methods.