Abstract:

15 Pigeonpea accessions have been studied by RAPD method in order to understand the genetic diversity of Pigeonpea. 27 random primers with higher polymorphism were screened out from plenty of random primers; 104 bands (59.24%) shows polymorphic among the total 176 RAPD identified bands. The amplified fragments have been counted and convert to data. The 15 Pigeonpea accessions have been divided into 6 classes according to the clustering result obtained from SPSS13.0 software. Meanwhile, 15 pigeonpea germplasms have planted in the field and recorded the biological characteristics. The clustering result in DNA level is similar to the classifying result with biological characteristics exhibited in field after comparing. The result provides valuable information for the utilization of genetic resources in pigeonpea germplasm.