Abstract:

Embryogenic callus of Citrus Sinensis L. Osbeck cv. Bingtang was used as initial explants to explore embryoid induction and multiplication. A majority of embryoids were malformed during the course of the multiplication, such as rosette, vitrification, albino embryoids or embryoids tended to recallus readily. Contents of part of soluble matters and activity of protective enzymes were compared in this study to investigate the factors induced malformed embryoids. The results showed that the soluble sugar and protein in the globular embryoids and embryoids tended to recallus readily were significantly higher than those of others, respectively, and the least contents were in the albino embryoids. For the SOD activity, the globular embryoids＞the rosette embryoids＞embryoids tended to recallus readily＞the vitrified embryoids＞the albino embryoids, whereas The POD activity of the vitrified embryoids was significantly higher than that of the globular embryoids and other types of malformed embryoids.