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Chinese Agricultural Science Bulletin ›› 2005, Vol. 21 ›› Issue (2): 54-54.

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The Technology of EST(Expressed Sequence Tag) and its Applications

Yu Fengchi   

  • Online:2005-02-05 Published:2005-02-05

Abstract: EST is a partial sequence of a group of cDNA and its normal length is 150~500bp. They were obtained from large-scale randomly sequencing of cDNA clones. One EST represents an expressed gene of one organism or cell at one time. Principle and method of EST technology and its applications, such as constructing genetic map, separating and identifying new genes, the research of expressed gene map, the research of comparing genomics, preparing DNA microarray were summarized in this article.