Abstract:

The main factors affected ISSR reaction were tested with Hongyu apricot as test materials to optimize ISSR amplification system, including the concentration of template, MgCl2, dNTPs and primer concentration as well as cycle numbers. The suitable ISSR amplification conditions of apricot were then conducted through the screening and optimization. The results showed that the optimal ISSR reaction was carried out in a volume of 25 μl containing 1 μl template DNA (40 ng), 2.5 μl dNTPS (2.5 mmol/L), 0.5 μl Taq DNA polymerase (2.5 U), 1 μl primer (20 μmmol/L), 2.5 μl 10×PCR Buffer, 2.5 μl MgCl2 (2.5 mmol/L), 15 μl ddH2O and 40 cycle numbers. The ISSR amplifications for 3 cultivars of common apricot were then investigated through the above reaction system. The system was completely suitable to analyze the genetic information for different apricot cultivars and thus supplied the further genetic diversity of apricot with theoretic bases and referenced value.