Abstract:

Endo-β-mannanase is an important enzyme involved in the mobilization of mannan, which is the component of hemicellulose. In order to investigate the relationship between endo-β-mannanase and organ abscission, the PCR based homologous cloning strategy was used to identify endo-β-mannanase (CiMan) genes from the fruit stalks abscission zone of navel orange (Citrus sinensis Osbeck). The results showed that a 274 bp cDNA encoding 91 amino acids was successfully cloned and characterized．Multiple alignment analysis of amino acid sequences of the putative CiMan cDNA fragment was 53%-65% homologous with other sequences from Genbank. It was supposed that the cDNA fragment was the endo-β-mannanase of navel orange.