Abstract:

The full-length cDNA sequence of elongation factor 2 (Mi-eft2) was obtained by RT-PCR and RACE from Meloidogyne incognita. It consisted of 2434 nucleotides, and encoded 777 amino acids. The amino acid sequence of deduced protein MI-EFT2 was 26.7%, 86.9 %, 13.8% and 8.9% identical with the deduced proteins of Ce-eft1, Ce-eft2, Ce-eft3 and Ce-eft4 from Caenorhabditis elegans respectively. Protein function prediction of MI-EFT2 suggested that it was a GTPase just like the eukaryotic elongation factor EF2, involved in the protein elongation process.