Abstract:

The Objective was to evaluate the composition of endophytic fungi groups of Balanophora harlandii Hook. f., and screen high inhibition activity substances from them. The endophytic fungi were isolated from surfaced-sterilized tissue, the inhibition activity substances were screened by agar well tests. 49 strains of endophytic fungi were isolated from Balanophora harlandii Hook. f.. 28 strains which grow well in PDA were classified into 7 different genera, 2 families, 2 orders through on their morphological characteristics. They were Rhizoctonia, Aspergillus, Penicillium, Geotrichum, Microsporum, Monilia and Oidium respectively. Antagonistic activities of the endophytic fungi against3 bacteria strains were tested in vitro. The cell-free filtrate of fermentation broth of strain SG47 showed notable inhibition effect on tested bacteria strains. The endophytic fungi isolated from Balanophora harlandii Hook. f. showed biological diversity, most of them had the ability to produce antibacterial activity substances. The plant endophytic fungi have a high potential to find novel pesticides or new medicines produced as secondary metabolites.