Abstract:

The same strain of Lactobacillus acidophilus(LA) were conservation by the different cryoprotectant and temperature , then take them a comparison in the time we preset. The groups of cryoprotectant we predetermined is that: DMSO-skimmed milk(1:9), DMSO-liquid MRS(1:9), glycerinum-skimmed milk(1:1), glycerinum-skimmed milk(8:2), glycerinum- normal saline(1:1), glycerinum-liquid MRS(1:1) and glycerinum-liquid MRS(8:2). With the extension of the conservation, the survival amount of the bacterial in the -80℃ group was more than that in the -20℃ group. The groups that had more number of survivorship of the Lactobacillus acidophilus than others had some denominators were that they had DMSO as cryoprotectant, skimmed milk or liquid MRS as preservation medium. The amount of survival LA was larger in this group than other groups, that was DMSO as cryoprotectant and with preservation medium as skimmed milk or liquid MRS.