Abstract:

DGGE-cloning sequencing method was chosen to study the effects of the planting of glyphosate resistant transgenic soybean (Roundup Reandy Soybean, RRS) on bacterial diversity in the rhizospheric soil during the growing period. The results showed that the rhizospheric bacterial 16S rDNA DGGE fingerprintings between glyphosate resistant transgenic soybean and non-transgenic soybean were similar within different growing stages, with only two different bands in the stages of emerging and feed filling, the two bands belonged to uncultured Gemmatimonadetes bacterium clone (vanished) and uncultured Firmicutes bacterium clone GASP-KC3W1_H04 (appeared) respectively; the diversity index and evenness index of 16S rDNA DGGE fingerprintings between glyphosate resistant transgenic soybean and non-transgenic soybean were with no significant differences. The glyphosate resistant transgenic soybean had no significant effects on the soil bacteria.