Abstract:

In order to explore the application of ISSR markers for molecular identification and marker-assisted breeding of Populus deltoides, 15 populus deltoides clones were analyzed by ISSR. ①The reaction volume of ISSR-PCR which was fit for populus deltoids was set up by single analysis of variance test. The reaction volume was 25 μL and adds with primer for 1.0 μmol/L, template for 30 ng, Taq enzyme for 1.5 U, dNTP for 0.25 mmol/L, Mg2+ for 2.0 mmol/L. ISSR program was: 3 min at 94℃ for calefaction to DNA denaturalized, then 35 cycles of 45 s at 94℃ for denaturalization, 30 s at 56℃ for annealing, 1 min at 72℃ for extension and extension at 72℃ for 5 min finally. ②15 populus deltoides clones were analyzed by 10 primers of ISSR. The number of the fragments examined were 63, the percentage of polymorphism fragments of each clone was between 20.63% and 30.16%, the highest of which were XL-77, XL-83, XL-101, 2KEN8 and I-69, the lowest of which were XL-92, XL-90. ③The clones except A65/31 belonged to the same group which included 3 subgroups, and the genetic differentiation and genetic relationship among the hybrid progenies of populus deltoides can be identified accurately by the specific makers bands of ISSR-PCR.