Abstract:

The homologous gene of Na(+)-dependent phosphate cotransporter (NaPi) in silkworm, BmNaPi2, was obtained through bioinfomatics analysis and molecular biological experiment. The gene is located on 3rd chromosome, 1 336 bp long (GenBank accession numer: HM593516), and 5 exons inside. The ORF of gene is 1 314 bp in length and encodes 437 amino acids, with 10 hydrophobic transmembrane domains. A comparison of BmNaPi2 with homologous protein from Drosophila melanogaster, Anopheles gambiae, Aedes aegypti, Culex quinquefasciatus, Tribolium castaneum revealed that their amino acids share about 57% similarity. It is shown by RT-PCR experiment that the BmNaPi2 has expression in blood, malpighian tubule and integument, while no expression in midgut, silk gland, gonad, head, and fat body among 9 tissues detected on day 3 of the fifth instar larvae.