Abstract:

HRM (high resolution melting analysis), which based on monitoring melting (dissociation) curves of double-strand DNA, is a recent advance for the detection of mutations and SNPs (single nucleotide polymorphisms). Due to its outstanding performance at sensitivity and accuracy, HRM has been largely used for mutation scanning, genotyping and DNA methylation research in recent years. In this paper, the mechanism and feature of HRM were introduced briefly; furthermore, the prospects of its application in plant breeding were provided by combining with the cases of HRM in analyzing plant populations.