Abstract:

【OBJECTIVE】This paper used Prunus Humilis leaves as experimental materials, the rate of polyphenols extraction indicators as the evaluation standard to explore the extraction process conditions of polyphenols in Prunus Humilis leaves.【METHOD】This paper had chosen acetone as extraction agent, and selected the extraction time﹑the solvent concentration﹑the liquid-solid ratio and the extraction temperature as four investigation factors, and used the L9(34) orthogonal experiment design, and used regular extraction method and microwave extraction method to study the best conditions of the extraction process of polyphenols of Prunus Humilis leaves.【RESULTS】The primary and secondary effects of polyphenols extraction indicators by using regular extraction method from Prunus Humilis leaves was as follows: extraction temperature>extraction time>solid-liquid ratio>extraction concentration; the optimal scheme was A1B3C3D3, which corresponded with the best treatment combination A1B3C3D3. The primary and secondary effects of polyphenols extraction indicators by using microwave method from Prunus Humilis leaves was as follows: extraction concentration>solid-liquid ratio>irradiation time>radiated power; the optimal scheme was A1B2C3D2, which did not correspond with the best treatment combination A1B2C2D2.【CONCLUSION】The best extraction process of polyphenols in Prunus Humilis leaves was the 70% of acetone solution, the solid-liquid ratio was 1:450, the extraction temperature was 70℃, the extraction time was 1.5 h。