Abstract:

【OBJECTIVE】The aim was to provide the theoretical basis for large scale propagation adventitious root of Oplpanax elatus Nakai.【METHOD】With the adventitious root of Oplpanax elatus Nakai as explants, the effect of concentrations of IBA, NAA and sucrose on adventitious root growth of Oplpanax elatus during suspension culture was examined in this study.【RESULTS】Auxin types and concentration affected adventitious roots growth, roots weights of fresh and dry were better in the medium supplanted with IBA than with NAA, IBA concentration of 3 or 4 mg/L was suitable for roots growth. Sucrose was also a important factor for adventitious roots growth, roots weights of fresh and dry were promoted by 50 g/L sucrose, and the values reached 5.3, 0.49 g respectively.【CONCLUSION】Adventitious root of Oplpanax elatus Nakai grew better when the formula of medium was MS+IBA 3 mg/L+50 g/L sucrose in suspension culture.