Abstract:

Trollius chinensis has been picked dramatically in rencent years, which has enormously destroyed the wild resources of the plant. Therefore, the introduction and domestication of the wild Trollius chinensis are important for landscaping and for the protection of the wild resources .In this study, the seedlings of Trollius chinensis were used as initial explant and Trollius chinensis’s culture in vitro were established. The effect of different time of sterilization on growth of seed was studied and the optimal medium composition for rooting and proliferation was selected by comparing with the effect of different medium culture on growing of Trollius chinensis. The results showed that: the seeds of Trollius chinensis was efficiently sterilized by 0.1% HgCl2 for 20 min; the optimal medium composition for proliferation of Trollius chinensis was 1/2MS+1.0 mg/L 6-BA+0.1 mg/L NAA; the optimal medium composition for rooting and seedlings of Trollius chinensis was 1/2MS or 1/2MS+0.05 mg/L NAA. The result provided the theoretical basis for the rapid propagation and production of Trollius chinensis on a large scale.