Abstract: The stem segments of Melaleuca bracteata was used as explants, the tissue culture and rapid propagation technology system was established. The results showed that the best initiated medium was MS+6-BA 1 mg/L, and the best proliferation medium was MS+6-BA 1 mg/L+NAA 0.1 mg/L+KT 0.1 mg/L. The optimum shoots rooting medium was 1/2MS＋IBA 0.1 mg/L, with the rooting of 100% and developed roots. The survival rate of tissue culture plantlets was over 89.3% after the plantlets acclimation transplanting experiment.