Abstract:

To establish a high frequency regeneration system for the gene transformation of soybean [Glycine max(L.) Merr.], cotyledonary nodes and embryonic tips from Heinong51 were used as the explants. Regeneration systems of cotyledon nodes and embryonic tips from Heinong51 were established in this research. Effect of 6-benzylaminopurine (6-BA) and indolebutyric acid (IBA) on soybean regeneration was studied. The results indicated that the optimal shooting induction medium of cotyledonary node from Heinong51 was MSB5 added 1.0 mg/L 6-BA and 0.2 mg/L IBA and the optimal rooting medium of cotyledonary node was MSB5 added and 1.0 mg/L IBA. The optimal shooting induction medium of embryonic tip from Heinong51 was MSB5 added 0.4 mg/L 6-BA and 0.2 mg/L IBA and the optimal rooting medium of embryonic tip was MSB5 added and 2.0 mg/L IBA. In comparison with regeneration system of embryonic tips, Heinong51 regeneration system of cotyledon nodes was superior in shoot regeneration frequency , shoot regeneration number and shoot elongation number. The cotyledon nodes from Heinong51 was more suitable for receptor materials in soybeans genetical transformation.