Abstract:

A rapid determination method was established by 16S rDNA sequence analysis via PCR amplification from the crude DNA extract of ‘Jinsi4’ jujube leaves with witches’ symptoms. The results showed that Jujube witches’ broom phytoplasma strain JWB-Jinsi4,TA shared the identity of 99.7% with Jujube witches’ broom phytoplasma strain JWB-G1（AB052876）and was classified as a member of 16Sr Ⅴ-B group, which indicated that PCR amplification method using crude DNA extract as the template could be used to detect Jujube witches’ broom phytoplasma, and provide the technique support for the diagnosis and control of jujube witches’ broom in productive practice.