Abstract:

The 1480 RAPD primers were used in the experiment. 26 flax materials from different countries were used for primer selection. 32 primers were selected which can amplify clear and reproducible polymorphic bands. The PCR results showed that the total of 79 polymorphic bands were amplified, the rate of polymorphic bands is 44.4%. The 32 random primers were used for genetic diversity analysis of 26 flax materials. The dendrogram was constructed based on genetic distances by using UPGMA method．The dendogram showed that 26 materials were clustered into 3 groups. The 10 core RAPD primers were selected from the 32 RAPD primers. They were S1047, S1230, S1238, S1270, S1314, S1353, S2105, SC07, SH04, ST09. The DNA fingerprints were constructed by the 10 core RAPD primers. There was a band as 1, there was not band as 0 in the fingerprints, and it constructed a digital fingerprint of 71 binary system numbers of one material. The ID cards of 26 flax materials were constructed by changing binary system into decimal system. The flax ID card system of RAPD marker was constructed primarily.