Abstract:

The aims were to detect ratoon stunting disease in virus-free Seedcane of sugarcane by real-time fluorescence quantitative PCR, establish and improve the real-time PCR technology system detection of healthy seedling of sugarcane ratoon stunting disease pathogen. Six sugarcanes ‘ROC22’ with ratoon stunting disease were selected, and the virus-free seedcane by the healthy seedlings production process of sugarcane were obtained. Real-time fluorescence PCR was used to detect the ratoon stunting disease pathogen gene expression of tissue culture samples. The results showed that the gel electrophoresis of 10 different treated seedlings sample PCR products didn’t have the target band, the average Ct value were from 37 to 39. Ratoon stunting disease pathogen genes did not express in 10 samples. Ten samples of healthy seedlings did not carry ratoon stunting pathogens. The Healthy seedlings in sugarcane production technology could remove the ratoon stunting disease pathogen thoroughly.