Abstract:

Folic acid, as the necessary nutrition of human, can defense many diseases, such as NDFS, so building up a rapid test method of folic acid is important to quality control of food or research in clinic. The Folic acid hapten was synthesized by Williamson synthesis method. immunogen and coating conjugate have been synthesized. Balb/c Mice were immunized with the folic acid conjugated with bovie serum avumin. The antiserum titer determined by indirect ELISA was more than 1.28×105，Using antibodies, the indirect competivive ELISA was established which was suitable for detecting FA: the IC50 was 14.28ng/mL.