Abstract:

In order to determine the optimum collecting time of GPC clean-up organophosphorous and pyrethroid residue in tobacco, and improve of purificatory effect and recoveries of those pesticide residues, the test was in progress. According to recovery tests for 16 organophosphorous and pyrethroid pesticide standard spiked samples with 3 concentration gradients, elution behavior of from a gel permeation chromatography column (GPC) was determined for these pesticides in tobacco leaf. The result showed that the optimum collection time of 9 organophosphorous pesticides from GPC column was 26-68 min and that for 7 pyrethroid pesticides was 26-42 min. The recoveries of organophosphorous pesticides were 80.28%-96.81%, the relative standard deviation were 4.44%-8.74%, the recoveries of pyrethroid pesticides were 76.39%-97.95%, the relative standard deviation were 2.69%-7.58%. The optimum collecting time of GPC clean-up pesticide residue is different from GB/T 13595, so the optimum collecting time must be determined by test.