Abstract:

To investigate tissue culture conditions to increase certain medicinal ingredients in tissue culture seedlings of Anoectochilus formosanus,using its tissue culture seedlings as the material,set four sucrose concentrations 1％,2%,3%,4％ and four treatments of subculture 10,30,50,70 days,then measured the content of polysaccharide and total alkaloids from its tissue culture seedlings after culturing for some time. The results showed that 4% of sucrose concentration was the most suitable quantity on the accumulation of polysaccharide;Polysaccharide content showed an increase after the first reduction with subculture days. Total alkaloid content decreased with the increase of sucrose concentration,and it was the highest with 1% of sucrose concentration. The content of total alkaloids was the highest after 50d days of subculture.